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Fig. 6 Alteration of hyaloid vasculature in larvae treated with MG-H1, which could be rescued by MG-H1 scavenger. (A) Scheme of experimental design for hyaloid vasculature exploration in larvae treated with MG-H1 with or without CAR rescue. Figure was created with BioRender.com. (B) Representative confocal images of the hyaloid vasculature showed amplified alterations in WT, GLO1KO and ALDH3A1KO larvae treated with 1 mM MG-H1, and attenuated alterations in larvae co-treated with 1 mM MG-H1 and 10 mM CAR. White scale bar = 20 μm. (C) Increased hyaloid branch points in WT, GLO1KO and ALDH3A1KO larvae treated with 1 mM MG-H1 was observed compared to the larvae without treatment, but no alteration was observed in DKO larvae treated with MG-H1, and these vasculature alterations can be rescued by CAR. n = 12–14. The bars indicate mean ± SD values. Statistical analysis was applied by one-way ANOVA, ns = not significant, **p < 0.01, ***p < 0.001. hpf, hours of post fertilization; dpf, days of post fertilization; w/o, with or without; MG-H1, methylglyoxal-derived hydroimidazolone 1; CAR, L-Carnosine.