ZFIN Image: Wu et al., 2025, FIGURE 7

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Figure Caption

FIGURE 7

ZEB1-Independent Effects of SB218078 on Angiogenesis. (A) SB218078 inhibits EMT-related morphological changes in MDA-MB-231 cells. (B) Immunofluorescence analysis of protein levels of Vimentin and E-cadherin in MDA-MB-231 cells treated with either vehicle control (DMSO) or SB218078 (1 μM and 2 μM). (C) Schematic representation of the designed gRNA sequences for zeb1a, snail1a, twist1a, and GFP. (D) RNA expression levels of each target in zebrafish across different treatment groups. (E–G) Knockout of ZEB1 using the CRISPR system inhibits the vascular development of intersegmental vessels (ISVs) and subintestinal vessels (SIVs) in zebrafish.

Acknowledgments

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