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Fig. 1 Experimental design showing the four fish species and two life stages of zebrafish used in the study on a logged time scale representing acclimation duration. Fishes were initially held at a control temperature representing ambient conditions during capture in the wild (16 °C: wrasse; 18 °C: stickleback and flounder) or rearing temperatures in the lab (28 °C: zebrafish) and then were transferred to a warm treatment of either +5 °C (wrasse, stickleback and flounder) or +6 °C (zebrafish). At each time point, CTmax was assessed for all species, and CTmax as well as CTmin were assessed for both life stages of zebrafish.