Fig. 8

Fig. 8 Combinatorial treatment with cysteamine and MitoTEMPO or liproxstatin-1 decreases 4-HNE protein expression in cystinosis podocytes. (a, b) Left panel: Representative western blot image of 4-hydroxynonenal (4-HNE)-conjugated protein levels in a. CTNS?/?#1 and b. CTNS?/?#2 treated with 100 µM cysteamine, 10 µM MitoTEMPO, 100 µM cysteamine?+?10 µM MitoTEMPO, 10 µM liproxstatin-1, or 100 µM cysteamine?+?10 µM liproxstatin-1 for 48 h. ?-Actin was used as a loading control. Right panel: Quantification of 4-HNE protein expression normalized to ?-Actin. (n?=?4 biological experiments, n?=?1 technical replicate) Statistical analysis: one-sample t-test, with WT set as the reference. AU?=?arbitrary units. Each dot represents a biological experiment