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Fig. 1 Protocol of EV isolation and zebrafish larvae development: a: Isolation protocol for whole zfl sEVs and lEVs by differential centrifugation. b-e: Zfl development; Imaging was performed at the 4 observation time points in 2% low-melting agarose (peqLab, Erlangen, Germany) using an Axio Observer microscope system (Zeiss, Jena, Germany), magnification 2,5x. b: 24 hpf, c: 48 hpf, d: 72 hpf, e: 96 hpf
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