ZFIN Image: Sebo et al., 2025, Fig. 7

Image

Figure Caption

Fig. 7 Activation of Wnt/?-catenin signaling in NPCs causes neuroinflammation. (a) Representative images of gfap:rtTA, TRE:?-cat*-EGFP, mfap:tdTomato larvae or wild type siblings (Con.) at 3 dpf, treated with indicated Dox concentrations. Magnified frames (5?�?Zoom) highlight microglia/macrophages in the hindbrain (Hb) and spinal cord (Sc). The CNS is outlined by yellow dotted boxes. (b, c) Quantification of mfap+ cells within the CNS (b) and in the whole larva (c) of larvae shown in (a) (n?=?6 for all conditions). p-values were calculated for comparisons of all groups using the parametric one-way analysis of variance (ANOVA) with Tukey HSD post hoc test (no label?=?not significant). Data are shown as mean?�?standard deviation. (d) Representative lateral images of gfap:rtTA, TRE:?-cat*-EGFP, mpx:mCherry larvae or wild type siblings (Con.) at 3 dpf, treated with indicated Dox concentrations. Magnified frames (5?�?Zoom) highlight neutrophils in the hindbrain (Hb) and spinal cord (Sc). The CNS is outlined by yellow dotted boxes. (e, f) Quantification of mpx+ cells within the CNS (e) and in the whole larva (f) of larvae shown in (d) (n?=?6 for all conditions). p-values were calculated for comparisons of all groups using the parametric one-way analysis of variance (ANOVA) with Tukey HSD post hoc test (no label?=?not significant). Data are shown as mean?�?standard deviation.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Sci. Rep.