ZFIN Image: Zhao et al., 2025, Fig. 5

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Figure Caption

Fig. 5 Fluorescence imaging and real-time quantification of EP in brain tissues and zebrafish.

a Fluorescence imaging and real-time quantification of EP in brain tissue and zebrafish. b Fluorescence images of brain tissue slices from S1BF, CA1, LD and Cpu regions incubated with CMG2 after electrical stimulation. Scale bar: 75 μm. c Representative traces of CMG2 in S1BF, CA1, LD and Cpu after electrical stimulation. d Dynamic response summary of CMG2 after electrical stimulation in S1BF, CA1, LD and Cpu (n = 15 independent experiments). e Fluorescence images of zebrafish incubated with CMG2 after electrical stimulation. Scale bar: 130 μm and 10 μm. f Representative traces of CMG2 in zebrafish after electrical stimulation. g Dynamic response summary of CMG2 after electrical stimulation in zebrafish (n = 15 independent experiments) The above-mentioned data are all presented as mean ± S.D. Error bars: S.D., gray dots represent individual data points. Statistical significance is calculated with a two-tailed unpaired t-test and P values are indicated (^nsp > 0.05, *p < 0.05, **p < 0.01, and ***p < 0.001). Source data are provided as a Source Data file.

Acknowledgments

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