Figure 9.

Figure 9.

Evaluation of single or combined drug-mediated effects on the induction of apoptosis in T47D BC cells. (a) The % of cells with active caspase-3 determined after PAX and EX527 treatment for 48 h using selected ratios of the IC₅₀ determined in the MTT assay. Data are presented as mean ± standard deviation (± SD) of the mean, *** p < 0.001 by one-way ANOVA, Tukey’s post-hoc test. (b) Representative dot plots from the FACS analysis of the T47D luminal-type BC cells after a 48h incubation with a medium PAX, EX527 or PAX:EX527 (1:1). R3-apoptotic cells with active caspase-3. (c) BAX relative gene expression in zebrafish T47D injected xenograft model after 72 h with 1/8 IC₅₀ EX527 or/and PAX treatment determined in qPCR method. *** p < 0.001 determined by one-way ANOVA, Tukey’s post-hoc test. (d) p21 relative expression in zebrafish T47D injected xenograft model after 72 h with 1/8 IC₅₀ EX527 or/and PAX treatment determined in qPCR method. *** p < 0.001 determined by one-way ANOVA, Tukey’s post-hoc test.