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Fig. 7 Graphic summary of the role of clec3bb in tendon ossification. A We discovered that myoseptal stem cell could differentiate into osteoblasts in wild type zebrafish by Slingshot analysis. Osteoblast2 further mineralized and formed IBs. Myoseptal stem cell could also differentiate into clec3bb + tenocyte, ultimately forming thbs4b + tenocyte. clec3bb showed specific expression in clec3bb + tenocytes. B After runx2b deficiency, the process of myoseptal stem cell differentiating into osteoblasts was inhibited. The process of differentiation from myoseptal stem cell to clec3bb + tenocytes undergone alterations. clec3bb expression level was increased in clec3bb + tenocytes of runx2b−/− zebrafish. C mRNA relative expression of biomarkers of exosome (cd81a, cd81b, cd9a, cd9b, tsg101, and cd63) in 15 dpf (stage without IBs/tendon ossification in wild type zebrafish) of runx2b−/− zebrafish and runx2b+/+ zebrafish and in 60 dpf (stage with IBs/tendon ossification in wild type zebrafish). D Comparison of gene expression between runx2b−/− and runx2b+/+ zebrafish (t-test, two-sided, *** (p value < 0.001)). Error bars represent mean ± SD. E The expression patterns of sp7, cd81b, and clec3bb gene in 60 dpf zebrafish by STARmap FISH