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Fig. 2 Single-cell RNA sequencing reveals the hematopoietic composition in WKM of adult wild type and rad21+/? zebrafish. A: uniform manifold projection (UMAP) showing the 22 cell clusters identified in scRNA-seq of WKM cells from wild type (WT) and rad21+/? adult zebrafish. B: stacked bar plot showing the percentage of annotated cell population within each genotype per replicate. WT1: 8,125 cells, WT2: 9,108 cells, rad21+/? 1: 6,159 cells, rad21+/? 2: 8,339 cells. C: fold change in population frequency in rad21+/? compared with WT. Red dot signifies statistically significant (P ? 0.05) change. Significance was determined using Student?s t test. D: violin plots of rad21, pcna, and myca expression in rad21+/? and WT. In rad21+/? versus WT comparison significance is from the scRNA-seq DESeq2 Wald test (Supplemental Table S3). *P < 0.05, ***P < 0.001, ****P < 0.0001. E: volcano plots showing DEGs identified in the comparison of rad21+/? versus WT in HSC-thromb and MPP clusters. Red dot signifies statistically significant (adjusted P ? 0.05) DEGs. F: GO enrichment analyses on up and down significant DEGs identified in MPP. Pathway analyses were performed using ClusterProfiler R package. Complete list of pathways and associated genes is provided in Supplemental Table S4. B, B-lymphocyte cells; E, erythroid; EP, erythroid progenitors; GMP, granulocytes monocyte progenitors; Gr, granulocytes; HSC, hematopoietic stem cell; M, monocytes/macrophages; MPP, multipotent progenitors; NK, natural killer; T, T-lymphocyte cells; thromb, thrombocytes; WKM, whole kidney marrow.