Figure 2

Figure 2

Skeletal muscle mass loss in zebrafish with type 2 diabetes mellitus (T2DM). (a) Hematoxylin and eosin (HE) staining of longitudinal sections of zebrafish tail skeletal muscle in each group, scale bar: 100 μm. The right side is an enlarged view of the red box on the left, scale bar: 20 μm. (b) Analysis of the total muscle fiber areas in each HE-stained section. (c) Analysis of the number of different muscle fiber areas in each HE-stained section. (d) Analysis of the difference in the muscle fiber area of the tail skeletal muscle in each group between 0 and 1500 μm² (small), 1501 and 3000 μm² (medium), and 3000 μm² and above (large). (e) EdU staining intensity of the skeletal muscle of zebrafish, indicating muscle proliferation and regeneration. EdU-labeled areas are indicated by the white arrows, scale bar: 500 μm. (f) Statistical chart of the positive area of EdU labeling. ** p < 0.01, *** p < 0.001. (n = 3 samples/group).