Figure 6

Figure 6

Deficiency of muc5AC increases the startle response latency. (A) Schematic diagram of the setup for vibration-induced startle response assay. The vibration module in Zebrabox is set to 70% maximum intensity and a frequency of 400 Hz. A high-speed camera is used to detect the movement of larvae. (B) Schematic diagram of the startle response experiment. T = 0 is the time when vibration stimuli is delivered. (C) Stages of the startle response of wild-type and muc5AC mutant larvae at 5 dpf. T = 0 represents stimulus onset. Arrowheads point to the detectable movement. Dashed rectangles highlight the whole process of startle response. T, ms. (D) Quantification of the short-latency startle response incidence rate in wild-type and muc5AC mutant larvae at 5 dpf. (E) Quantification of the short-latency startle response time in wild-type and muc5AC mutant larvae at 5 dpf. (F) Quantification of the startle response duration time in wild-type and muc5AC mutant larvae at 5 dpf. ***, p < 0.001; ns, not significant.