ZFIN Image: Ma et al., 2025, Figure 1

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Figure Caption

Figure 1

Structure, multiple alignment, and phylogenetic tree of Mucin-5AC proteins. (A) Schematic diagram of Mucin-5AC domains in zebrafish, little skate, spotted gar, medaka, and human. SP, signal peptide; CysD, cysteine rich domain; PTS, central proline, threonine, serine (PTS)-rich sequence. Green bars represent von Willebrand domains. (B) Phylogenetic tree of the first or second CysD domains of Mucin-5ACs. (C) Multiple alignment of the first or second CysD domain of Mucin-5ACs. Asterisks represent conserved cysteines (C). Black boxes indicate conserved serines (S) and threonines (T). (D) Secondary structure element of CysD1 in zebrafish Mucin-5AC. (E) Amino-acid sequence arrangement of CysD1 in zebrafish Mucin-5AC. The blue arrows (β1–β6) represent anti-parallel beta-strands and the yellow cylinders (α1–α2) represent short alpha helices. The black lines (SS1–SS2) mark disulfide bonds. (F) Three-dimensional structure of zebrafish Mucin-5AC (CysD1-CysD4) predicted by Alphafold.

Acknowledgments

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