ZFIN Image: Carra et al., 2024, Figure 8

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Figure Caption

Figure 8

(a) Example of the profiles of the cross section of the light sheet image acquired by means of a reflecting mirror (640 × 512 pixels, 880 µm × 700 µm). The inset reports the image of the sheet cross-section on which the dashed lines indicate only a few of the sections that are analyzed in the main panel. The lines in the main panel are the best fit of the profiles to a Gaussian function with an average fitting FWHM = 7.8 ± 1.3 µm. (b) Example of the average cross-section of a single 1 μm fluorescent bead imaged in the light sheet used to determine the value of the PSF of the setup (640 × 512 pixels, 880 × 700 µm²). The dashed line is the best fit Gaussian function to the data with FWHM = 3.2±0.4 μm. The inset shows an example of the image of a microsphere in the light sheet.

Acknowledgments

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