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ZDB-IMAGE-250110-111

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Méjécase et al., 2024 - Oxidative Stress, Inflammation and Altered Glucose Metabolism Contribute to the Retinal Phenotype in the Choroideremia Zebrafish

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Figure 4

PFKM overexpression improves retinal phenotype but not photoreceptor cell death at 5 dpf. (a) Human PFKM expression was detected 5 days post-injection in chm^ru848 and wt zebrafish. (b) mRNA-injected chm^ru848 showed a more preserved photoreceptor layer (white asterisk) with better retinal lamination and a thicker RPE layer compared to the uninjected mutants (n = 9 zebrafish per group). Scale bar: 50 μm; scale bar zoom in: 20 μm. (c) Cell viability is unchanged after PFKM overexpression in wt and chm^ru848 zebrafish (n = 9 zebrafish per group). Scale bar = 50 μm. ONL: outer nuclear layer; INL: inner nuclear layer. (d,e) Expression of anti-apoptotic (d) and pro-apoptotic (e) markers were analysed using RT-qPCR at 5 dpf in zebrafish eyes, from uninjected and injected wt and chm^ru848. Data are expressed as mean ± SEM from n = 3 (with 20 eyes per group). Statistical significance was determined by one-way ANOVA. * p < 0.05; ** p < 0.005; **** p < 0.0001. “●”—wt; “▲”—wt + PFKM; “▼”—chm^ru848; “◆”—chm^ru848 + PFKM.

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Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Antioxidants (Basel)