Figure 4

Figure 4

IGS activates mTORC1 pathway in acinar cells

(A and C) Representative images of pancreas immunofluorescence from the 2 mouse models. Green, amylase; red, phosphor-S6 (240/244); blue, DAPI.

(B and D) Quantification of pS6 intensity in Gcg−/− mice (B) or antibody-treated mice (D) and their controls (n = 5/group). The intensity was normalized to control mice or the IgG treatment group. ∗p < 0.05, ∗∗p < 0.01, Student’s t test.

(E) Schematic experimental design for treating mice with sirolimus (rapamycin) treatment.

(F) Relative pancreas weight in the four groups (n = 5–6/group).

(G) Representative immunofluorescence images of acinar tissues. Amylase, green; Ki67, red; DAPI, blue. Arrows point to Ki67+ acinar cells.

(H) Quantification of Ki67+ acinar cells in the four groups (n = 5/group). Scale bar, 100 μm (A and C), 50 μm (G). Data are represented as mean ± SEM. ∗p < 0.05, ∗∗p < 0.01, ∗∗∗p < 0.001. One-way ANOVA followed by Turkey’s Multiple Comparisons Test.