Fig 3

Fig 3 Locomotor defects and brain cell death in developing zebrafish following ZIKV infection.

(A) Schematic experimental setup and behavioral analysis (created with BioRender.com). (B) Representative swimming tracks of control (mock), ZIKV-infected (ZIKV), and ZIKV-infected and NITD008-treated (ZIKV+NITD008 (100 μM)) larvae at 4 days post-fertilization. (C) The distance moved by the larvae was assessed using the DanioVision device (Mock, n = 40; ZIKV, n = 45; ZIKV+NITD008 (100 μM), n = 30. N = 3). Data are shown as median ± 95 CI. **** P ≤ 0.0001; Kruskal-Wallis test. (D) TUNEL staining at 2 days post-fertilization showing cell death in the developing brain following injection in zebrafish embryos. A = anterior; P = posterior. Scale bars = 50 μm. (E) The number of TUNEL+ cells was quantified (Mock, n = 15, N = 3; ZIKV normal, n = 20, N = 3; ZIKV mild/severe, n = 26, N = 3; ZIKV+NITD008 (100 μM), n = 14, N = 2). Data are shown as median ± 95 CI. **** P ≤ 0.0001; Kruskal-Wallis test. n indicates the number of fish; N represents the number of independent experimental repeats.