Fig 2

Fig 2 ZIKV injection results in viral protein accumulation in larval brain.

(A) At 3 dpf, mock- and ZIKV-infected larvae were fixed and subjected to whole mount immunostaining with anti-NS3 antibodies. Representative pictures of 4 imaged samples are shown. The arrows indicate specific ZIKV NS3 signals. The schematic representation of the zebrafish larva illustrating the region of interest was created with BioRender.com. A = anterior; P = posterior. (B) Schematic representation of a zebrafish brain at 4 days post-fertilization. The forebrain is shown. The gray line represents the localization of the transverse section. Th and PTh are areas of the diencephalon, a division of the forebrain. Dien = diencephalon; Tel = telencephalon; Th = thalamus; PTh = prethalamus; Ve = ventricle; A = anterior; P = posterior; D = dorsal; V = ventral. (C) Transverse brain cryo-sections of 4 days post-fertilization mock-injected or ZIKV-injected larvae are shown. Sections were stained with anti-ZIKV E antibody (green) and analyzed by confocal microscopy. Nuclei were labeled with Hoechst. Images are maximal intensity projections. Scale bars = 50 μm.