Figure 2

Figure 2

Comparison of dio3b and thrb crispants at retinal level. (A) Electropherograms from a fragment of the PCR amplicon sequence for dio3b and thrb of the vehicle-microinjected controls and the corresponding crispants. At the top, the sgRNA sequence is denoted, and the PAM sequence is highlighted in red. In the crispants, a decrease in the resolution of the electropherogram is evident around the canonical cutting site indicated with an arrow and a dotted line. (B) Representative histological cross-sections of retinas of vehicle-injected controls and thrb and dio3 crispants (5–7 retinas per condition) stained with H&E. (C) Amplification of the GCL (insets in (B)). (D) Quantification of the number of cells/1000 µm². ONL = outer nuclear layer, INL = inner nuclear layer, GCL = ganglion cell layer. Statistical analyses were performed using one-way ANOVA and Tukey post hoc tests. * = p < 0.05. Scale bar = 20 µm.