Fig. 1

Fig. 1

Metastatic conjunctival melanoma cells induce angiogenesis and recruitment of macrophages in a zebrafish angiogenesis model. a Schematic representation of the angiogenesis assay in zebrafish xenografts. Cancer cells or 2% PVP used as vehicle are injected into the PVS at 2 day post fertilization (dpf) zebrafish larvae Tg(kdrl:EGFP^s843; mpeg1:GAL4-VP16^gl24; UAS-E1b: NfsB-mCherryⁱ¹⁴⁹). In response to a strong angiogenic signal, the SIV complex is deformed towards the injection focus. b Representative images of time-lapses recording the angiogenic response to the SIV from 2 to 24 hpi. c Quantifications of the angiogenic activity induced by engraftment of CoM cells at 24hpi as measured by the angle, total length, and elongation of the SIV complex