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Fig. 4 Knockdown of the Pnck gene induces antitumor immune response. (a) Volcano plot showing DEGs in the sequencing results, including upregulation of genes encoding immune cell chemotactic factors, like Ccl24, Ccr10, Vtcn1, Ackr4 and Cxcr2. Differentially expressed genes were identified with the threshold of |log2(fold change)| >1 and false discovery rate (FDR)<0.05. (b) Heatmap showing the enrichment and upregulation of immune-related pathways in the Pnck-KD group. (c) GSEA enrichment analysis of complement and cytokine-related pathways in the PNCK low-expression group, and GSEA enrichment of cell cycle and DNA repair-related pathways in the high-expression group. (d) Immunohistochemistry demonstrated the expression levels of CD4, CD8, and CD163 in PNCK high and low groups. (e) Infiltration abundance of CD8 T cells in mouse tumors in the WT and Pnck-KD group (n=3 biologically independent samples).