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Fig. 2 T2 relaxation time and ADC measurement in various brain regions of control and tlr2 mutant (tlr2−/−) zebrafish. (A) Anatomical DWI (b-value 1000 s/mm2), sagittal slice, showing ROIs used for T2 and ADC estimation. (B) Estimated T2 relaxation times in selected ROIs of control and tlr2−/− zebrafish. (C) Estimated ADC in selected ROIs of control and tlr2−/− zebrafish. Acquisition details for MSME: TR 3000, τ 6.0 ms, 4 averages, resolution 47 × 47 μm and a slice thickness of 500 μm; and for DWI: TR 1000 ms, TE 13 ms, 4 averages, resolution 47 × 47 μm, slice thickness 200 μm and effective b-value range 50, 500, 1000, 1500, 2000, 2500 or 3000 s/mm2. Statistical analysis was performed using the unpaired t-test, assuming Gaussian distribution, with p less than 0.05 considered to imply significant differences between the control and the tlr2−/− group. *p < 0.05, **p < 0.01, ****p < 0.001. ADC, apparent diffusion coefficient; CCeg, granular layer of the cerebellar corpus; CCem, molecular layer of the cerebellar corpus; DIL, diffuse nucleus of the inferior lobe; DM, medial zone of the dorsal telencephalon; DWI, diffusion-weighted imaging; MLF, medial longitudinal fascicle; MSME, multislice multiecho; ROI, region of interest; TL, longitudinal torus.