Figure 3.

Figure 3. Generation of stable mutant naa80 knockout lines. (A) Overview of naa80 mutant zebrafish crossing and experiments performed. (B) The Naa80 protein with location of 5del/1in and 13del mutations, as well as locations of the Ac-CoA binding motif (red) and proline-rich loop (yellow) highlighted. Also highlighted are the sense and missense regions and the premature stop codons which arise in the mutant alleles. (C) Representative PCR products (112 bp from WT allele, 99 bp from 13del allele) from naa80 13del fin clips visualized in agarose gel (4% agarose in lithium borate acetate buffer and GelRed).