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Fig. 7 Zebrafish mRpL4 modulates expression of Notch signaling components during development A. Expression of notch1a, hey1, and her15.1 mRNA in mrpl4‐null larvae comparing to wild‐type control at 5 dpf as measured by quantitative PCR (three biological replicates for each genotype and three technical replicates for each sample). Statistical significance was tested using two‐tailed unpaired t‐test. Error bars represent ± SD; **P < 0.01, ***P < 0.001. B. Representative bright field image of 5 dpf wild‐type larva (n > 30) illustrated by in situ hybridization with a mrpl4 riboprobe. C. Representative bright field image of 5 dpf CTRL (n = 26/29) and mrpl4 mutant (n = 12/15) illustrated by in situ hybridization with a notch1a riboprobe. D. Representative bright field images of 5 dpf CTRL (n = 28/30) and mrpl4 mutant (n = 10/12) illustrated by in situ hybridization with a hey1 riboprobe.