Fig 1

Fig 1 MYCN overexpression in SAP cells does not increase their cell numbers during early larval stages.

A) Cartoon model of a 3 dpf dbh:EGFP or dbh:EGFP-MYCN larval fish, depicting imaging regions and selection of the SCG as the region of interest (ROI). B) Quantification of cells detected at 3, 4, 5, and 6 dpf after “spots” detection using IMARIS. For 3 dpf EGFP n = 15, EGFP-MYCN n = 14; for 4 dpf EGFP n = 15, EGFP-MYCN n = 15; for 5 dpf EGFP n = 13, EGFP-MYCN n = 9; and for 6 dpf EGFP n = 9, EGFP-MYCN n = 7. C-L) Representative images of dbh:EGFP (C-G) or dbh:EGFP-MYCN (H-L) larvae from 3 to 6 dpf. Boxed region represents the SCG. ROI from 3, 4, 5, and 6 dpf larvae is shown in full detail (D-G, H-L), with the cells detected after performing the spots pipeline in IMARIS (D’-G’, H’-L’). A (anterior), P (posterior), D (dorsal), V (ventral) axes shown in upper right corner. hb = developing hindbrain, sc = developing spinal cord, cg = developing cranial ganglia, scg = developing superior cervical ganglia, y = yolk. Scale bars = 100 μm for full size image, 25 μm cropped images. n.s., non-significant (P>0.05).