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Fig. 5 Overexpression of Notch3 intracellular (N3ICD) but not extracellular (N3ECD) domain rescues gliogenesis in scarb2a mutants.a?d? Dorsal views and transverse optical sections of Tg(olig2:EGFP) hindbrain in wt (a, a?), scarb2a?/? (b, b?) and scarb2a?/? following heat-shock induced overexpression of Notch3 extracellular (N3ECD) (c?c?) and Notch3 intracellular (N3ICD) domains (d?d') (dashed square in a?d, marks the region shown in a??d?). e Quantification of migrating OPCs in a?d (n = 9 embryos, One-way ANOVA, multiple comparisons with Tukey post-hoc test, PANOVA < 0.0001). f?i? Dorsal views and transverse optical sections of Tg(slc1a3b:MYRGFP-2A-H2AmCherry) hindbrains in wt (f), scarb2a?/? (g) and scarb2a?/? mutant following N3ECD (h-h?) or N3ICD (i?i?), heat-shock induction (dashed square in f?i, marks the region shown in f??i?) j, Quantification of slc1a3b+ astrocytes in f?i (n = 9 embryos, One-way ANOVA, multiple comparisons with Tukey posthoc test, PANOVA < 0.0001). Error bars are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not statistically significant. Scale bars, a?, b?, c?, d?, f?, g?, h?, i? = 30 ?m; a, b, c, d, f, g, h, i = 60 ?m.Source data are provided as a Source Data file.