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Fig. 3 scarb2a depletion leads to impaired gliogenesis.a, b?, Dorsal views (a, b) and transverse optical sections (a?, b?) of Tg(olig2:EGFP) wt (a, a?) and scarb2a?/? (b, b?) at 60 hpf showing significant reduction of olig2+ migrating cells and in the pMN domain (dashed lines) in scarb2a mutants. (dashed squares in a, b mark the level of the optical sections showed in a?, b?). c Quantification of olig2+ migrating OPCs (n = 14 embryos, two-tailed Student?s t-test, P < 0.0001) and d olig2+ OPCs in the pMN domain (embryos n = 14 embryos, two-tailed Student?s t-test, P < 0.0001). e?f?, Dorsal views (e, f) and transverse optical sections (e?,f?) of Tg(mbp:EGFP) embryos at 5 dpf depicting reduced numbers of myelinating OLs in scarb2a?/?, quantified in (g) (n = 14 embryos, two-tailed Student?s t-test, P < 0.0001). h?i? Dorsal views (h, i) and transverse optical sections (h?, i?) of Tg(slc1a3b:MYRGFP-2A-H2AmCherry) x wt (h, h?) and scarb2a?/? (i, i?) showing significantly fewer astrocyte nuclei (red) in the vz of mutant hindbrains at 60 hpf (dashed squares in h, i mark the level of the optical sections shown in h?, i?), quantified in j (n = 11 embryos, two-tailed Student?s t-test, P < 0.0001). k?l', Dorsal views (k, l) and transverse optical sections (k', l') of Tg(slc1a3b:MYRGFP-2A-H2AmCherry) embryos at 6 dpf showing reduced numbers of astrocytes, quantified in m (n = 10 embryos, two-tailed Student?s t-test, P < 0.0001). n?p Dorsal views of Tg(olig2:EGFP;GFAP:Gal4FF) hindbrain in wt (n), scarb2a?/? (o), and scarb2a?/? mutant following injection of UAS-scarb2a-P2A-RFP (p). q Quantification of migrating olig2+ cells (n = 13 embryos; One-way ANOVA, multiple comparisons with Tukey posthoc test). r?t Dorsal views of Tg(slc1a3b:MYRGFP-2A-H2AmCherry;GFAP:Gal4FF) hindbrains in wt (r), scarb2a?/? (s) and scarb2a?/? mutant following injection of UAS-scarb2a,cmcl2:EGFP (t). u Quantification of slc1a3b+ red nuclei (n = 12 embryos; One-way ANOVA, multiple comparisons with Tukey posthoc test). Error bars are mean ± s.e.m. *P < 0.05, **P < 0.01, ***P < 0.001, ****P < 0.0001; ns, not statistically significant. Scale bars, a, b, e, f, h, i, k, l, n?p, r?t = 50 ?m; a', b', e', f?, h', i', k', l' = 30 ?m. Source data are provided as a Source Data file.