Fig. 6

Fig. 6 Peds1a/b DKO line shows developmental delay, neutropenia, monocytopenia and exacerbated inflammation.

A Generation of a DKO line by whole deletion of peds1a and peds1b genes using CRISPR-Cas9. B–F Total plasmalogen and precursor quantification (B), larval length (C), number of neutrophil (D) and macrophage (E), and transcript levels of nfkb1, il1b, tnfa and clxcl8a genes, assayed by RT-qPCR (F). Each point represents one larva and the mean ± SEM of each group is also shown. *p ≤ 0.05, **p ≤ 0.01, ***p ≤ 0.001. ROI region of interest, CHT caudal hematopoietic tissue.