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Fig. 1 Effect of valproic acid (VPA) on zebrafish larval survival and hatching rates at up to 120 h post-fertilisation (hpf) (n = 120 per group). (A) Kaplan–Meier survival comparison in WT-AB fish showed a significant effect [log-rank (Mantel–Cox) test] of VPA treatment at 100 (p ≤ 0.05), 250, and 500 μM (p ≤ 0.0001) as compared with untreated controls (CTRL) as early as 24 hpf. (B) The hatching rate of WT-AB fish was significantly affected [log-rank (Mantel–Cox) test] by VPA treatment with doses of 100 (p ≤ 0.05), 250, and 500 μM (p ≤ 0.0001) at 72 hpf. (C,D) Survival and hatching rates of Tg(mpeg1:EGFP) transgenic fish were not influenced by any tested treatment (p > 0.05).