Figure 3

Figure 3

Effect of diazepam (DZP) on PILO-induced local field potential (LFP) events. Larvae were incubated in medium containing DZP (20 μM) for 20 h, and, subsequently, PILO was added to wells (final concentration = 50 mM). After 5 min incubation with PILO, LFPs recording were performed: (A) control recording of seizure-like events as presented on the graph evoked by PILO 50 mM; (B) representative LFP recordings performed in larvae exposed to DZP 20 μM + PILO 50 mM, note that in 3 out of 4 larvae, no seizure-like activity was recorded within 20 min of analysis, (C) only 2 short-lasting, low amplitude seizure-like events were found in larvae no. 3 within 20 min of analysis.