Figure 2.

Figure 2. Transcriptomic changes in hepatocytes after β-cell ablation.

(A) Schema showing the experimental design to identify transcriptional changes in hepatocytes after β-cell ablation in 2-mo-old zebrafish. (B) Glucose measurements of the 2-mo-old zebrafish used for hepatocyte isolation. Measurements were made before liver dissection. n = 5–9. A Mann–Whitney test was used to assess significance. **P = 0.0045. (C) Log₂ fold change plot showing the up-regulated and down-regulated genes in hepatocytes after β-cell ablation. The significantly differentially expressed genes (P adj < 0.05) are highlighted as red dots. (C, D, E, F) Statistical overrepresentation analysis of the down-regulated genes from (C) using the PantherDB tool. (D, E, F) Fold enrichment of the significantly enriched processes (D), molecular function (E), and Reactome pathways (F) is shown.