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Fig. 3 KO of tmem106bb impairs definitive hematopoiesis. (A) Appearance of embryos showed increased red blood cells in tmem106bb?/? embryos at 3 dpf (n = 24?27 embryos per group; scale bar: 200 ?m). The black arrowheads indicate red blood cells. (B) O-dianisidine staining showed a significantly increased signal for red blood cells in tmem106bb?/? embryos at 3 dpf (n = 15?18 embryos per group; scale bar: 200 ?m). The black arrowheads indicate red blood cells. (C) WISH for mpx showed that myeloid differentiation was significantly increased in tmem106bb?/? embryos at 3 dpf (n = 19?20 embryos per group; scale bar: 200 ?m). (D) WISH for lplastin showed that myeloid differentiation was significantly increased in tmem106bb?/? embryos at 3 dpf (n = 17?26 embryos per group; scale bar: 200 ?m). (E) WISH for gata1 showed that erythroid differentiation was significantly increased in tmem106bb?/? embryos at 4 dpf (n = 16-embryos per group; scale bar: 200 ?m). (F) WISH for hbbe1 showed that erythroid differentiation was significantly increased in tmem106bb?/? embryos at 4 dpf (n = 16?20 embryos per group; scale bar: 200 ?m). (G) WISH for rag1 showed that lymphoid differentiation was significantly reduced in tmem106bb?/? embryos at 5 dpf (n = 16?26 embryos per group; scale bar: 200 ?m). ***p < .001; Data were shown as mean ± SD; Statistical analysis was performed using a Shapiro?Wilk test for samples with normal distribution and unpaired student's t tests with two tails.