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Fig. 2 drish expression pattern analysis in zebrafish by whole-mount in situ hybridization. (A) drish was restricted to the nervous system (white asterisk) in the head at 5-somite (11.5 hpf) stage. (B–D) At the 11-somite (14.5 hpf) stage, drish was expressed in the forebrain including the eye field (yellow asterisk), anterior cephalic mesoderm (ACM), midbrain and hindbrain (marked by a bracket), endothelial progenitor cells (EPCs), and neural plate (NP). (E) At 18 hpf, drish was broadly expressed in the cranial neural region, while it was restricted to vascular endothelial cells (arrowhead) and the spinal cord (arrow) in the trunk. (F, G) drish expression was absent from vascular endothelial cells in etv2−/− mutants (arrowhead). (H) At 24 hpf, drish was expressed in the brain, spinal cord (arrow), and the dorsal aorta (arrowhead). (I–L) By 28 and 35 hpf, drish was primarily expressed in the brain and spinal cord (arrow, L). Note drish expression in the brain (arrowheads) adjacent to the retinal pigmented epithelium (RPE). Scale bars: 100 μm. Scale bar in E for E-G. Lateral views in all panels, except for (C, D), which is a flat-mounted embryo, dorsal view, and (J, K), which are dorsal views. (M) Analysis of drish expression in the previously published Daniocell single-cell RNA-seq dataset.42 Only cell types with any significant drish expression are shown. Note its strong expression in retinal progenitors/Müller glia precursors at 14–21 hpf stages. The figure was made using Daniocell analysis tool https://daniocell.nichd.nih.gov/ and subsequently edited.