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Fig. 4 PDX1 and MC4R energy metabolism regulation. (a) Comparison of convergent amino acid substitutions in the PDX1 and MC4R genes across 15 fish genomes. (b) Functional verification of the convergence site in PDX1. Plasmids containing fixed point mutations in the PDX1 gene were injected into fish eggs, and the consumption of yolk was calculated (n = 20). Analysis was performed using ANOVA, and the resulting p-value is denoted as (*p-value < .05, **p-value < .01, *** p-value < .001, ****p-value < .0001). (c) PDX1 and MC4R genes are involved in insulin (INS) regulation.
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