ZFIN Image: Gutsfeld et al., 2024, Fig. 4

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Figure Caption

Fig. 4 Experimental design for exploratory RNA-seq. Head dissections of PFOS (0.88μ⁢M, 1.57μ⁢M, 2.8μ⁢M) or PFHxS (7.87μ⁢M, 14μ⁢M, 25.1μ⁢M)-exposed larvae were conducted on 4–5 dpf for RNA-seq with five biological replicates per condition. Differentially expressed genes (DEGs) were used for functional enrichment analysis using ToxNodes in SOM-format, calculation of transcriptomic benchmark concentrations (BMCT), and upstream regulator predictions via an Ingenuity Pathway Analysis. Note: dpf, days postfertilization; PFHxS, perfluorohexanesulfonic acid; PFOS, perfluorooctanesulfonic acid; RNA-seq, RNA sequencing; SOM, self-organizing map.

Acknowledgments

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