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Figure Caption
Fig. 5 Directing protein localization with split-mNG2. A. Schematic illustrating use of the split-mNG2 system to sequester proteins of interest on mitochondria. B–G. Representative images of krt8-mNG211 embryos injected with mNG21-10 (B–D) or mito-mNG21-10 (E–G) mRNA and stained with MitoTracker dye to label mitochondria. Maximum projections of confocal z-stacks. Arrows indicate colocalization between split-mNG2 and MitoTracker fluorescence. Images were acquired from the tail fin epidermis at 48 h post-fertilization (hpf). Scale bars, 50 μm.
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Reprinted from Developmental Biology, 514, Ligunas, G.D., Paniagua, G., LaBelle, J., Ramos-Martinez, A., Shen, K., Gerlt, E.H., Aguilar, K., Nguyen, N., Materna, S.C., Woo, S., Tissue-specific and endogenous protein labeling with split fluorescent proteins, 109-116, Copyright (2024) with permission from Elsevier. Full text @ Dev. Biol.