ZFIN Image: Mashayekhi et al., 2024, Fig. 1

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Figure Caption

Fig. 1 Characterization of HCT116 parental and KO cell-derived EVs isolated via the TFF system. A, B Representative NTA size distribution profiles of isolated EVs. C, D NanoSight quantification of EV preparations' size (C) and concentration (D) are shown as mean ± SD, n = 3 individual EV isolation, triplicates). E Cryo-TEM images of EVs, scale bar: 500 nm. F Abundance of 25 vesicle markers in WT and KO cells and EVs. Values are shown as log2 signal intensity of three independent preparations. G Cellular internalization of EVs into primary macrophages (1:10,000 cell:EV). Cells were incubated for 24 h with WT and KO EVs labeled with CFSE. Magnification, 63x, scale bar: 10 µm

Acknowledgments

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