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Fig. 2 Binding affinity of CTX-AMFA and TRZ-AMFA for M6PR and their target. (A) Binding affinity for M6PR. The affinity of CTX or CTX-AMFA (left) and TRZ or TRZ-AMFA (right) for M6PR was determined by ELISA. Results are presented as the absorbance of mAb or mAb-AMFA bound to M6PR and detected with HRP-conjugated anti-human IgG antibody. Non-specific adsorption values of the antibodies bound to uncoated wells are subtracted. (B) Antigen affinity. The affinity of CTX or CTX-AMFA (left) and TRZ or TRZ-AMFA (right) for EGFR and HER2, respectively, was determined by ELISA. Results are presented as the absorbance of mAb or mAb-AMFA detected by HRP-conjugated anti-human IgG antibody (mean ± SEM from triplicates).