Figure 4

Figure 4

Knockout of ace and morphological examination of ace^−/− mutant larvae. (A) The generation of ace mutations in zebrafish through CRISPR/Cas9 technology. (a). The targeted exon 8 of ace containing the knockout site. Exons are indicated by boxes, blue box means coding region of exon, while white box means non-coding region of exon, introns are represented by fold lines. The red letter in the wild type (WT) means the position some changes will happen, while the dotted line and blue letter corresponded to red letters means deletion and transition happened in the mutant (MT), respectively. (b,c). In comparison to the wild type, the mutants had a deletion of 7 nucleotides in exon 8, leading to skipped mutation (red letters), and premature termination of translation at the 468th amino acid. The asterisk in the figure indicates the position of protein translation termination. (d). To assess the impact of ace knockout, RT-PCR was performed to analyze ace expression in both ace^−/− and wild-type samples (B) Expression patterns of fabp2 in ace^−/− mutants and wild-type larvae at 5 dpf, as detected by WISH. (C) The morphology of the intestinal epithelium in ace^−/− mutants and wild-type larvae at 5 dpf, as shown by HE staining.