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Fig. 5 Visualization of the cranial bone region of 5 dpf zebrafish larvae by calcein staining. Zebrafish larvae (2 dpf) were treated with PEG at 1 μg/ml and 10 μg/ml for 3 days. At 5 dpf, zebrafish larvae were stained with 0.2% Calcein solution for 10 min. The fluorescent signal of calcein was observed using fluorescence microscopy. Side view of the cranial bone region marked with white contour. A Representative bright-field image of zebrafish larvae at 5 dpf (scale bar 240 µm). B–D No significant differences were observed between untreated and PEG-treated larvae (scale bar 500 µm)
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Full text @ J. Endocrinol. Invest.