Figure 3

Figure 3

Functional effect of verapamil on the insulin content and glucose stimulated-insulin secretion (GSIS) rate of MIN6 cells. (A) Measured insulin content in MIN6 cells treated with 50 µM verapamil (red) for 24 h or left untreated (blue). (B) Total secreted insulin by MIN6 cells and (C) total insulin content in MIN6 cells when stimulated with different concentrations of glucose and either treated with 50 µM verapamil (red) for 24 h or left untreated (blue). (D) Percentage of secreted insulin over total insulin content of each condition. Each experiment was performed in 4 independent repeats. Difference between the groups was statistically analyzed using two-way analysis of variance (ANOVA), and p-values < 0.05 were considered statistically significant. Data are presented as mean ± SEM; * p-value < 0.05; ** p-value < 0.01; *** p-value < 0.001; and **** p-value < 0.0001, ns: non-significant.