IMAGE

Figure 3

ID

ZDB-IMAGE-240620-179

Antibodies

Ab1-adamtsl4

Publication

Tevar et al., 2024 - The Increased Burden of Rare Variants in Four Matrix Metalloproteinase-Related Genes in Childhood Glaucoma Suggests a Complex Genetic Inheritance of the Disease

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Figures for Tevar et al., 2024

Image

Figure Caption

Figure 3

Localization of ADAMTSL4 protein in zebrafish eye larvae (6 dpf) by confocal fluorescence immunohistochemistry. Fluorescent immunohistochemistry was performed on 10 μm histological sections of zebrafish eyes from wild type (+/+) (A) and adamtsl4 KO (−/−) (B) specimens. The sections were incubated with a rabbit anti-ADAMTSL4 primary antibody (MBS716409, Quimigen) at a dilution of 1:250, followed by a Cy2-conjugated donkey anti-rabbit secondary antibody at a dilution of 1:1000. As a negative control (C), a section was incubated only with the secondary antibody. In the resulting images, ADAMTSL4 immunoreactivity is represented by green signals, DAPI nuclear staining by blue signals, and tissue autofluorescence by red signals. Scale bars in the panels indicate 50 μm. The images are representative of the observed results in three zebrafish of each genotype. C: cornea. GCL: ganglion cell layer. INL: inner nuclear layer. IPL: inner plexiform layer. LEP: lens epithelium. ONL: outer nuclear layer. OPL: outer plexiform layer. PHL: photoreceptor layer. POM: periocular mesenchyme.

Figure Data

Expression / Labeling details

Phenotype details

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ Int. J. Mol. Sci.