Fig. 5

Fig. 5

mTOR knock out affected mitochondrial morphology and membrane potential. (A) Mito-Tracker red staining of mTOR-WT and mTOR-KO larval zebrafish fin following amputation. (B) Representative transmission electron microscope images of mTOR-WT and mTOR-KO larval zebrafish fin after amputation. (C-E) Statistical analysis of the average area, aspect ratio and average width of mitochondria between mTOR-WT and mTOR-KO larval zebrafish fin after amputation. (F-G) The ROS levels between mTOR-WT and mTOR-KO larval zebrafish fin after amputation. (H-I) Measurement of the mitochondrial membrane potential by JC-1 staining between mTOR-WT and mTOR-KO zebrafish at 48 hpa. (J) Location of mitochondrial fission related gene (dnm1l) in mTOR-WT and mTOR-KO larval zebrafish fin by in situ hybridization. (K) mRNA expression level of dnm1l in mTOR-WT and mTOR-KO larval zebrafish fin. *P < 0.05, **P < 0.01