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Fig. 6 ulk1a mutants repair defective autophagic flux in both embryonic and adult stages of ttntv-A mutants. (A) Schematics of ulk1ae6 generated by MMEJ sgRNA. Red indicates deleted nucleotides. * indicates the nonsense codons. (B and C) Quantification of the autophagosome puncta in 2 dpf embryos either with or without BafA1 treatment and the autolysosome/autophagosome ratio of BafA1-untreated groups. ulk1a+/– can reduce the basal autophagosome level of dA/dA and can recover the autophagic flux affected by dA/dA. One-way ANOVA was applied. (D–F) Representative Western blot and quantification of ulk1a and atg7 from 3-month zebrafish hearts. One-way ANOVA was applied. (G and H) Representative Western blot and quantification of LC3-II from zebrafish hearts either with or without BafA1 treatment at 3 months. Independent samples t test was applied to compare 2 groups. Data are presented as mean ± SD.