Figure 12

Figure 12

Comparative analysis of CNCC proliferation in cdc27^−/−, cdkn1a, and tp53 knockout embryos at 48 h post-fertilization (hpf). This figure displays the results of immunofluorescence staining using antiphosphohistone H3 (PH3), a marker of cell proliferation, in three groups of zebrafish embryos: cdc27^−/− mutants, cdkn1a knockouts, and tp53 knockouts. The merged images highlight anti-PH3 signals within the CNCCs in the pharyngeal arch region, marked by a dotted box. Notably, the intensity of these signals in cdkn1a and tp53 knockout embryos is increased compared to the cdc27^−/− embryos, indicating a higher rate of cell proliferation in the pharyngeal arches of these knockouts. This suggests a variation in the proliferation rates of CNCCs due to different genetic modifications, with cdkn1a and tp53 knockouts exhibiting enhanced proliferation relative to the cdc27^−/− mutants. Dotted box: pharyngeal arch reign. Note: ****, p < 0.0001.