ZFIN Image: Wang et al., 2024, Fig. EV1

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Figure Caption

Fig. EV1 Elevated ROS levels in HEK293 stable transfected cell lines expressing NOX4 variants. HEK293 cells were subjected to 12 h of serum starvation after stably transfection with following plasmids: pcDNA3.1, NOX4wt, NOX4Y512IfsX20, NOX4Y512C, and NOX4V369F. Fluorescence imaging was conducted following the incubation with 10 ?M DCFH-DA. Representative photomicrographs of the fluorescence are displayed and quantification of the mean fluorescence intensity was performed with ImageJ software. N = 3. Scale bars: 100 ?m. Data information: DCFH-DA 2?,7?-dichlorofluorescein diacetate. Data presented as mean � SD. N = biological replicates. The P value was calculated by the ordinary one-way ANOVA multiple comparisons with Turkey correction of multiple hypothesis tests. Source data are available online for this figure.

Acknowledgments

This image is the copyrighted work of the attributed author or publisher, and ZFIN has permission only to display this image to its users. Additional permissions should be obtained from the applicable author or publisher of the image. Full text @ EMBO Mol. Med.