ZFIN Image: Jiang et al., 2024, Figure 2

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Figure 2

ACL attenuated the inhibition effect of FAC in bone formation in zebrafish. (A) Calcein staining of zebrafish larvae (7 dpf) cranium from control, FAC (200 µg/mL), FAC with low (0.1 mg/L) or high (0.5 mg/L) concentration ACL treatment groups. (B) Mineralized areas were quantified and shown in the graph. (C) The number of calcein-stained vertebra bodies were calculated. (D–F) The relative mRNA expression of spp1, bglap and runx2a in zebrafish larvae treated with blank medium, FAC, FAC + high (0.5 mg/L) or low (0.1 mg/L) ACL were determined by qPCR. The mean value of blank medium-only treated group was set to 1.0. Data are showed as mean ± standard error. * means p < 0.05 between two indicated groups (n = 6). M, Meckel; PQ, palatoquadrate; CH, ceratohy; HY, hyomandibula (hyosympletic); OP, opercula.

Acknowledgments

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