ACL exposure caused developmental malformations in zebrafish at high concentrations. (A) The chemical structure of ACL. (B) Locomotion tracks, (C) average speed and (D) total movement distance of zebrafish larvae exposed to 0.01–10 mg/L ACL treatment at 5 dpf (n = 25). (E) Zebrafish embryos survival proportions treated with 0.01–10 mg/L ACL for 10 days. (F) Malformation phenotypes of embryos exposed to 0.01–10 mg/L ACL for 3–5 days. (G) Malformation rate of different concentrations of ACL-treated embryos in 5 dpf (n = 50). (H) Cell viability of MC3T3-E1 cells under various concentrations of ACL for 24 h or 48 h, blank was treated as the control group. Data are expressed as the mean ± standard deviation (SD), * p < 0.05 versus the control group.
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