Fig 2

Fig 2 RGC axon termination is disrupted in gsx1^y689.

(A-C) Max projections of confocal z-stacks through the optic tectum from start of Tg(atoh7:EGFP) (green, RGC axons) labeling to end in (A-Aⁱⁱ) wildtypes (~85μm), (B-Bⁱⁱ)gsx1^y689/+ (~82μm), and (C-Cⁱⁱ)gsx1 mutants (~80μm). Tg(slc17a6b:DsRed) labels glutamatergic neurons (magenta), and white arrowhead, Pr, points to pretectal region lacking Tg(slc17a6b:DsRed) expression in gsx1^y689. Schematic in (A) displays lateral orientation with dashed circle indicating removed eye. Scalebar = 100μm. (Aⁱⁱⁱ-A^iv, Bⁱⁱⁱ-B^iv, Cⁱⁱⁱ-C^iv) Depth coded view of same samples in A-Cⁱⁱ to provide reference for some AF regions with color coordinated arrowheads for depth, AF9 = dark blue, AF7 = yellow, AF3 = orange, AF6 = yellow. (A^iv, B^iv, C^iv) Zoomed in view of the pretectal AFs from dashed outline box in Aⁱⁱⁱ, Bⁱⁱⁱ, Cⁱⁱⁱ. Scalebar = 50μm. AF9 can be seen in blue across each genotype, while AF7 is absent in gsx1^y689 as seen by loss of yellow depth indicator.