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Fig 2

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ZDB-IMAGE-240429-101
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Figures for Schmidt et al., 2024
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Figure Caption

Fig 2 RGC axon termination is disrupted in gsx1y689.

(A-C) Max projections of confocal z-stacks through the optic tectum from start of Tg(atoh7:EGFP) (green, RGC axons) labeling to end in (A-Aii) wildtypes (~85μm), (B-Bii)gsx1y689/+ (~82μm), and (C-Cii)gsx1 mutants (~80μm). Tg(slc17a6b:DsRed) labels glutamatergic neurons (magenta), and white arrowhead, Pr, points to pretectal region lacking Tg(slc17a6b:DsRed) expression in gsx1y689. Schematic in (A) displays lateral orientation with dashed circle indicating removed eye. Scalebar = 100μm. (Aiii-Aiv, Biii-Biv, Ciii-Civ) Depth coded view of same samples in A-Cii to provide reference for some AF regions with color coordinated arrowheads for depth, AF9 = dark blue, AF7 = yellow, AF3 = orange, AF6 = yellow. (Aiv, Biv, Civ) Zoomed in view of the pretectal AFs from dashed outline box in Aiii, Biii, Ciii. Scalebar = 50μm. AF9 can be seen in blue across each genotype, while AF7 is absent in gsx1y689 as seen by loss of yellow depth indicator.

Acknowledgments
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