Figure 5

Figure 5

In vivo efficacy of BVZ-AMFA2_(n=5) in zebrafish angiogenesis model. Tg(fli1:eGFP) zebrafish embryos were injected intravenously with 15 ng BVZ or BVZ-AMFA2_(n=5) at 48 hours post fertilization. Zebrafish embryos were observed at 0, 24 and 48 h post-injection under confocal microscope. The representative fluorescence images of control (n=8) and embryos treated with BVZ (n=11) or BVZ-AMFA2_(n=5) (n=11) at 48 h post-injection are presented for the subintestinal vessels (SIVs) area, and the blood vessels are indicated by arrows (A). The SIVs area (B) and the number of blood vessels (C) were determined, and the results are expressed as the angiogenesis rate compared to the value at T0 or as the number of blood vessels, respectively. Tukey’s test: * p value < 0.05; ** p value < 0.01.